Abstract
Purpose
To investigate the association between proteomic changes and potential pathogenesis
in the human cornea with respect to the duration of wearing soft contact lenses (SCLs).
Methods
A total of 96 corneal stroma samples, obtained via small incision lenticule extraction
(SMILE), were equally grouped according to the duration of wearing SCL: 0Y, did not
wear SCL; 5Y, wore SCL for<5 years; 5–10Y, wore SCL for 5–10 years; O10Y, wore SCL
for>10 years. Liquid chromatography–tandem mass spectrometry was used to identify
and quantify protein profiles in the corneal stroma. Expression levels of CO1A1, CO4A1,
NFKB1, and IL6RB were determined using western blot and immunohistochemistry analysis.
Results
This study quantified a total of 5,668 proteins across samples and identified 2,379
differentially expressed proteins (DEPs) with significantly increased abundance in
the three SCL-wearing groups compared with that in the non-SCL-wearing group. Compared
with those in the 0Y group, the molecular functions of DEPs in the 5Y, 5–10Y, and
O10Y groups were mainly related to translation regulator activity, antigen binding,
peptidase inhibitor activity, participation in extracellular matrix (ECM) production,
complement activation, and inflammatory responses. Pathway enrichment analysis of
DEPs showed that the sphingolipid, phosphatidylinositol 3-kinase-protein kinase B,
and hypoxia-inducible factor-1 signaling pathways were activated in the human corneal
stroma after long-term SCL use.
Conclusions
Inflammation-related proteomic components in human corneal stroma increased after
long-term use of SCL and may act as an essential factor in the molecular pathogenesis
of corneal stroma damage.
Keywords
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Article info
Publication history
Published online: February 10, 2023
Accepted:
January 29,
2023
Received in revised form:
December 26,
2022
Received:
October 4,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2023 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.
