Abstract
Purpose
This aim of this study was to assess anti-parasitic properties of deep eutectic solvents
against eye pathogen, Acanthamoeba, often associated with the use of contact lens.
Methods
Assays were performed to investigate the effects of various Methyltrioctylammonium
chloride-based deep eutectic solvents on Acanthamoeba castellanii, comprising amoebicidal assays, encystment assays, excystment assays, cytotoxicity
assays by measuring lactate dehydrogenase release from human cells, and cytopathogenicity
assays to determine parasite-mediated host cell death.
Results
In a 2 h incubation period, DES-B, DES-C, DES-D, and DES-E exhibited up to 85 % amoebicidal
activity at micromolar doses, which was enhanced further following 24 h incubation.
When tested in encystment assays, selected deep eutectic solvents abolished cyst formation
and were able to block excystment of A. castellanii. All solvents exhibited minimal human cell cytotoxicity except DES-D. Finally, all
tested deep eutectic solvents inhibited amoeba-mediated cytopathogenicity, except
DES-B.
Conclusions
Deep eutectic solvents show potent antiamoebic effects. These findings are promising
and could lead to the development of novel contact lens disinfectants, as well as
opening several avenues to explore the molecular mechanisms, various doses and incubation
periods, and use of different bases against Acanthamoeba castellanii.
Keywords
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Article info
Publication history
Published online: October 13, 2022
Accepted:
September 9,
2022
Received in revised form:
September 6,
2022
Received:
March 13,
2022
Identification
Copyright
© 2022 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.
