Highlights
- •Cosmetic contact lens that dislodge pigments tend to adhere P. aeruginosa.
- •P. aeruginosa of cytotoxic genotype are more resistant to certain disinfectant solutions.
- •Type III secretion system is involved in P. aeruginosa disinfectant sensitivity.
Abstract
Purpose
To compare the sensitivity of two genotypes of P. aeruginosa to various disinfectant solutions and analyze the attached bacteria on worn cosmetic
contact lenses (cosCLs).
Methods
In this prospective study, healthy volunteers wore etafilcon (brown), nelfilcon (gray),
or hilafilcon (black) cosCLs and microbial adhesion analysis was performed. A rub-off
test determined pigment dislodgement. Disinfectant sensitivity to Optifree Replenish
(Alcon), Optifree Pure Moist (Alcon), Renu Fresh (Bausch & Lomb), and AoSept Plus
(Ciba Vision) was tested at various disinfection times and compared between various
genotypes and Type III secretion (T3S) system mutants.
Results
Of the 1152 cosCLs collected, 364 were culture positive (32%). The highest rate of
culture-positive lens was hilafilcon (chi square, P = 0.0001). Hilafilcon also had
a significantly greater number of isolates than etafilcon (P < 0.0001). Hilafilcon
was the only lens to fail the rub-off test. Cytotoxic strains were significant more
resistant to Renu Fresh than were invasive strains, even at 100% of recommended disinfection
time (P = 0.0005). Of the tested disinfectants, Renu Fresh was significantly less
effective in killing both genotypes of P. aeruginosa compared to AoSept Plus at all time points (25%, 50%, 75%, and 100% recommended disinfection
time, P = 0.0001, 0.0001, 0.0005, and 0.0005, respectively). When the T3S system was
dysfunctional, mutant strains were all susceptible to disinfectants (P = 0.0001 for
both invasive and cytotoxic strains).
Conclusion
Pseudomonas species is commonly found on cosCLs of asymptomatic individuals. Wearers
of cosCLs that dislodge pigments may be predisposed to microbial contamination. Cytotoxic
strains are more resistant to disinfectant solutions, especially to Renu Fresh. P. aeruginosa disinfectant resistance requires a functional T3S system.
Keywords
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Article info
Publication history
Published online: November 09, 2019
Accepted:
October 27,
2019
Received in revised form:
October 15,
2019
Received:
May 28,
2019
Footnotes
☆Presented in part at the annual meeting of the Association for Research in Vision and Ophthalmology, Denver, Colorado, USA, May 3–7, 2015.
Identification
Copyright
© 2019 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.
