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Inflammatory proteins associated with contact lens-related dry eye

  • Author Footnotes
    1 These authors contributed equally to this work and are co-first authors.
    Padmapriya Ramamoorthy
    Footnotes
    1 These authors contributed equally to this work and are co-first authors.
    Affiliations
    Alcon Laboratories, USA
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  • Author Footnotes
    1 These authors contributed equally to this work and are co-first authors.
    Safal Khanal
    Footnotes
    1 These authors contributed equally to this work and are co-first authors.
    Affiliations
    Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham, Birmingham, AL, USA
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  • Jason J. Nichols
    Correspondence
    Corresponding author at: University of Alabama at Birmingham, 1716 University Blvd, Birmingham, AL, 35223, USA.
    Affiliations
    Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham, Birmingham, AL, USA
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this work and are co-first authors.
Published:April 03, 2021DOI:https://doi.org/10.1016/j.clae.2021.101442

      Abstract

      Purpose

      To evaluate the levels and regulation of tear film inflammatory proteins in contact lens-related dry eye (CLDE).

      Methods

      One hundred healthy, daily wear (non-overnight), experienced soft contact lens wearers were classified into normal (n = 50) and CLDE (n = 50) groups based on Contact Lens and Dry Eye Questionnaire scores, tear break-up times, and comfort (a two-hour difference between total and comfortable daily lens wear hours). Tear samples (up to 5 μL) were collected by capillary extraction from the inferior meniscus of each eye, and pooled tear samples (10 per group) were tested using a customized Quantibody array. Mann Whitney tests with the Benjamini-Hochberg procedure with a 5% false discovery rate were used to compare the normal and CLDE groups.

      Results

      Relative to the normal group, the CLDE group showed a significantly increased tear concentration of several inflammatory mediators, including interleukin (IL)-7 (p = 0.001), IL-8 (p = 0.001), IL-13 (p = 0.001), IL-15 (p = 0.001), IL-12 p70 (p = 0.002), growth-related oncogene-alpha/ chemokine (C X C motif) ligand 1 (p = 0.003), granulocyte–colony stimulating factor (p = 0.005), IL-11 (p = 0.008), epidermal growth factor receptor (p = 0.01), IL-1 receptor antagonist (RA) (p = 0.013), macrophage colony-stimulating factor (p = 0.013), Eotaxin/C C motif chemokine ligand 11 (CCL11) (p = 0.016), and IL-2 (p = 0.016). The following cytokines were increased three-fold or more in the CLDE group: IL-13 (p = 0.001), Eotaxin/CCL11 (p = 0.016), and IL-1RA (p = 0.013).

      Conclusions

      Several inflammatory markers, including interleukins, were increased in tears of subjects with CLDE. These results support a growing body of evidence that suggests a potential role of inflammation in CLDE.

      Keywords

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